BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. Choose from a three- (M3), four- (M4), orThere were 12 replicate wells per dilution in both 96-well and 384- well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Fluorescence Photometric Performance (M2) Dual monochromators: 1 nm increments: EX: 250-850 nm: EM: 250-850 nm: Bandwidth (EX, EM). 6 % of reading 4. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. . The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. 6 % of reading 4. The SpectraMax M2 reader can be easily converted and optimized for very low-throughput to medium-high throughput analysis, faster, more accurate results and saving reagents. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. Standard features include a cuvette port,. Temperature control range from Ambient +4º to 50ºC. 00. Plate chamber design of the SpectraMax category 1 microplate readers. 7. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Allows you to choose your sample format depending on your unique research and throughput needs. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. MPN: 50505. Single-Label Plate Reader: SpectraMax Plus 384 (Molecular Devices). Appendix. Important things to consider when purchasing a multimode microplate reader. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). A). Type Clear: Add to Cart. Published Dec 16, 2020 | Updated Dec 16, 2020. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Figure 2. When the user applies a set temperature of 37°C, the top is heated to 37. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Used. spr SpectraMax Plus 384. D. Bioz Stars score: 86/100, based on 1 PubMed citations. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. S. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. In the M2, M5 and Plus. SpectraMax ® M2 Multi-Mode Microplate Reader. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. 40266 Email: drugdiscoverytech@lifetech. 1) Select the correct plate and the wells that you want to measure. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. SpectraMax ® M2 Multi-Mode Microplate Reader. 627. (a) Chemical structures of 1 (HADP) and its analogues 2–4. 5 x 38. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. (a) Chemical structures of 1 (HADP) and its analogues 2–4. duAl monochromAtors With SpectraMax M2/M2e. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Add to Cart. Multimode Microplate Readers. Molecular devices SpectraMax M5 Pdf User Manuals. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. free tracer decreases, resulting in an overall increase in RFU values. Usage/Application: Lab. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. SpectraMax Microplate Readers. USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. Multimode Readers Comparison Chart. Microplate Readers. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). D 3 1. Multimode microplate readers deliver both performance and economy. Type Clear: Add to Cart. Model: SpectraMax M2. 0077 OD 0. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Perkin Elmer 1420-2560 Victor X Series Plate Reader. 1 New Reader Support 1. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. A solution for low volume applications. The SPECTRAmax GEMINI XS Microplate Spectrofluorometer from Molecular Devices is a microplate reader capable of detecting signal from either fluorescence or luminescence based assays. 00 CAD. We therefore gathered salient. The SpectraMax® M2 and M2e Multimode. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. Add to Cart. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. g. This plate reader is capable of monitoring UV and visible absorbencies, fluorescence, and luminescence. 0 × 103 to 2. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Specialized microplate readers for luminescence detection are available for use in applications such as luciferase reporter assays, bioluminescence resonance energy transfer (BRET), and other luminescent assays. Customer Satisfaction is. fluorescence intensity (Figure 6). Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. The following general steps are taken with our absorbance and fluorescence readers. Plate reader set to 50 reads. FI assay. 25 to 3. Perform the scan and note the wavelength that gives maximum emission. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single 1) Select the correct plate and the wells that you want to measure. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. was 1. of each sample medium was transferred to a 96-well flat-bottom plate. To schedule a training session please contact Ewa Folta-Stogniew. I am wondering if the type of 96-well plate matters. made for this assay plate. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. View online or download Molecular devices SpectraMax M5 User Manual. An approach to gain setting that I like to use is to set the gain based on the sample with the most fluorescence in order to get about 30-50% of the maximal signal before the detector saturates. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. (b) Fluorescence responses of probe HADP and control compounds. Qualify the fluorescence performance of the SpectraMax . With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax Glo Steady-Luc Reporter Assay Kit. Fluorescence measurements no longer require you to adjust the gain. Offer Ends In: 40 Days 05: 02: 03. spr SpectraMax iD5 SpectraTest ABS2 v1. For example, microplates containing different samp les can be read using the same orThe plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. If you are going to read it from the bottom, you should use a clear-bottom black plate. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. 00. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. spr SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. 67. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The calculated LOQ values were 0. Endpoint, kinetic, spectrum and area-well. Perkin Elmer Envision. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. These robust readers have been placed in labs from Antarctica to the. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 2. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Multimode Readers Comparison Chart. 0 out of 5 $ 3,249. It does not work as expected. As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. Appendix A. It can measure absorbance from 230 -1000 nm. The chamber contains. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. B. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Microplate Reader Shelf. Read the microplate Step 1: Place the microplate in the reader. 1. 119. The SpectraMax iD3 reader detects fluorescence using monochromators only. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. It allows you to explore cellular pathways and protein activation and expression in one system. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. e. BioTek Synergy 2. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. Refurbished. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. In the lower half of the window, left side, there is a box labeled reader. 25 to 3. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Downloads. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. I have been using the Molecular Devices SPECTRAmax GEMINI XS for the last 6 months, mostly in time-resolved fluorescence,. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Standard. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. A solution for low volume applications. Temperature variation ± 0. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. SoftMax Pro. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 2. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Plate Readers. Absorbance (monochromator). 0 × 107) RLU 0. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. SpectraMax Quant dsDNA Assay Kits. Reviews. 8 Customer Reviews. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. For a higher degree of automation, the Automation. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Temperature Control: ambient from +4 to 45C. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. Microplate Reader, For Lab. Warranty: 60. patent nos. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Compatible automation solutions for SpectraMax Readers. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The plate reader. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Microplate readers’ standard detection modes are. • Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In addition, the SpectraMax iD5SpectraMax Microplate Readers. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The chamber. Microplate Readers Biotek Synergy 2 Plate Reader Multi-Mode. 0) OD (0. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Note: In this user guide, SpectraMax ® refers to several SpectraMax . S. SpectraMax ® M2/M2e: Not Capable: Validated:. Tecan’s. These measurements can include parameters such as absorbance, fluorescence intensity and. The Infinite® M1000 from TECAN is a high-end multimode Microplate Reader with premium Quad4 Monochromators. Featuring temperature control up toThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Molecular Devices SpectraMax M2 ; Molecular Devices. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. The fluorescence was measured in the SpectraMax M3 Microplate reader. 7. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices SpectraMax M2 Multimode Plate Reader. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Filters: Excitation and emission slides for 4 filters each. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Type: Microplate Reader. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Incubation. When reading optical density at wavelengths below 340 nm, special UVtransparent,. An assay plate containing a 15-point standard curve was read on the SpectraMax. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. 5°C at 37°C. (See Figure 7. Calcein AM is a widely used live-cell marker. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. CA$14,500. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. 49 µM,. 1. Fully tested with validations and warranty. 1 SoftMax Pro v4. D 47 7. Data from The SpectraMax® iD3 Multi Mode Microplate . The password is. 2. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Molecular Devices Spectra Max Gemini XS fluorescence Microplate reader. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. 00. 2. Data from slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Measure more accurately. 0077 OD 0. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Features. 2 only) SpectraMax M-series Microplate readers. Fluorescence Microplate Readers 4. With optimized reagents, option to operate the readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Settings for SpectraMax iD3 Multi-Mode Microplate Reader. D 47 7. Fill a clean plate with water. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax® Microplate Readers and SoftMax® Pro Software. Features. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Adjusting fluorescence gain on microplate readers. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. When a feature or capability applies to only certain readers, this exception is noted. 1 x 38. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. Figure 6: Transcreener ADP2 FI assay principle. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. (a) Chemical structures of 1 (HADP) and its analogues 2–4. 40266 Email: drugdiscoverytech@lifetech. 4. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 2018. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. The Cut-off filter for this pair is showing 530nm in this machine. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. SpectraMax M2 and use thereof is covered by issued (U. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. SpectraMax M2, M2e;. 8: theMicroplate Reader & Luminometer Selection Guide. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. STAKMAX. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. PerkinElmer’s new EnSight Multimode Plate Reader reads label-free Corning® Epic® technology (in addition to standard labeling technologies) and is appropriate when multiple assay types are more important than speed. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax Microplate Readers. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Fully tested with validations and warranty. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. FI assay. NanoQuant plate. type: Clear: Add to Cart. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Instructions. The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. $16,000. 10. Add to Cart. The kit includes everything you need: reagents, buffers and a microplate. Product Information. assays, we can offer a microplate reader solution to help meet the. . UsethecuvetteportforAbsorbance,Fluorescence,andLuminescence(secondary mode)readmodes. VANTAstar™ Flexible Multi-mode Microplate Reader. 0) OD (500 to 1100) RFU (5. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Appendix A. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. Molecular Devices SpectraMax M2. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. 2. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. The fluorometric. both cuvette-based and microplate. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Similar settings will work for other SpectraMax readers with fluorescence detection. The flexibility of a high end multimode plate reader with environmental control. Maximum signal with lowest background. 25 to 3. The SpectraMax i3 Multi-Mode Microplate Reader and the SpectraMax MiniMax 300 Imaging Cytometer enable both ways of assessing transfection conditions for GFPbased reporter assays. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. 0 out of 5 $ 3,895. D 3 1. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax. Linear, Orbital Slow, and Orbital Fast. Download PDF. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 67.